Lung puncture biopsy is the gold standard for diagnosing lung cancer, and if cancerous tissue is detected, lung cancer is basically diagnosed. Lung cancer is mainly characterized by clinical symptoms such as cough, chest pain, hemoptysis or blood in sputum, fever, etc. When lung cancer is suspected by combining clinical manifestations and imaging manifestations, biopsy can be performed. Lung puncture biopsy is usually performed under the guidance of x-ray fluoroscopy, b-ultrasound or chest ct. Commonly used methods include thoracoscopy, bronchoscopy, transthoracic puncture for lung biopsy, etc. It is less traumatic, more convenient to operate, and able to obtain the results quickly, and it is mainly applicable to tumors in the close proximity of chest wall. If tumor cells are found in the biopsy, the diagnosis of cancer can be confirmed. The biopsy can also determine the pathological type of lung cancer, so as to guide the selection of treatment plan and prognosis judgment afterwards. It is recommended that patients actively cooperate with doctors to improve relevant examinations, and actively treat the tumor after clear diagnosis to avoid delaying the condition.