The best time to check sex hormones is related to the patient’s status and gender. Most female patients are recommended to check on the 2nd-3rd day of menstruation, while amenorrhea and more urgent female or male patients can check at any time without any special time restriction. The 2nd-3rd day of menstruation belongs to the early follicular stage, during which the secretion of various hormones is relatively low, which can reflect the true state of ovarian function. It is often recommended that female patients check their sex hormones during this period, especially on the 2nd-3rd day of menstruation. However, if the patient’s condition is more critical, it is necessary to go to the emergency department or endocrinology department of the hospital in time to avoid delaying the condition. In addition, there is no special time requirement for male patients and amenorrheic female patients to check their sex hormones. In order to ensure the accuracy of the results, the test should be performed on an empty stomach and no strenuous activities should be performed before the blood is drawn. During the test, you need to inform the doctor what period of your menstruation you are in, as there are different reference ranges for hormone levels during different periods, which is beneficial to the doctor’s diagnosis of your condition.