Acrosomal enzymes are present on the inner membrane of the sperm acrosome and the equatorial part of the membrane, usually in an inactive form, and are activated as acrosomal enzymes when the sperm head enters the egg zona pellucida. This enzyme is an indispensable neutral protein hydrolase in the fertilization process, and its action is similar to that of trypsin. It hydrolyzes the egg zona pellucida glycoprotein, allowing the sperm to cross the egg mound and then the zona pellucida, allowing the sperm to fuse with the egg; it also promotes the release of kinins in the reproductive tract, thereby enhancing sperm motility and promoting spermatozoa movement. Acrosome enzymes are indispensable for sperm motility and fertilization, and insufficient acrosome enzyme activity can lead to male infertility. Therefore, measurement of sperm acrosome enzyme activity can be used as a reference indicator for sperm fertilization ability and diagnosis of male infertility.