How long it takes for antibodies to become negative with Lyme disease treatment is generally uncertain, as positive antibodies can be false positives and have no diagnostic value. It should be analyzed on a case-by-case basis. 1. The false-positive rate of IgG antibody test for Lyme disease can be as high as 27.5%, which has no significance on whether the antibody turns negative after treatment for this group of patients, and it may not turn negative after treatment. the IgM test is only applicable to the early stage of the disease <4 weeks. 2. Cerebrospinal fluid examination of patients with Lyme disease after the use of large amounts of antibiotics may still show a prolonged increase in antibody levels, even up to 10 years; Therefore, whether Lyme disease is cured cannot be judged by whether the antibodies have turned negative or not, and repeat testing or treatment is not necessary if the patient has no new symptoms. Further treatment is indicated for patients with a history of Lyme disease and persistent clinical symptoms. Early medical consultation and regular treatment under the guidance of a physician are recommended.