Anti-Jo-1 antibodies are one of the anti-ENA antibodies and are mostly considered as marker antibodies in polymyositis and dermatomyositis. Patients with these antibodies often present with a specific group of syndromes such as myositis combined with interstitial lung lesions, symmetric arthritis, mechanic’s hand, Raynaud’s phenomenon, and fever, which are called anti-Jo-1 antibody syndrome. When this antibody is present, it is especially important to investigate myositis and polymyositis, dermatomyositis, and laboratory tests will reveal elevated biochemical myosin, and chest CT will reveal interstitial lung lesions and symmetric arthritis. The mechanic’s hands are like the hands of many factory workers, very rough and some of them have cracks. There are also some cases of Raynaud’s phenomenon, in which the hands turn white, then purple, and finally red when exposed to cold or stress. There may also be fever, as anti-Jo-1 antibody syndrome is one of the rheumatic immune diseases.