First generation, second generation and third generation IVF, their indications are different, as follows: 1. First generation IVF, that is, conventional IVF, is mainly for women with poor fallopian tubes or several times of IUI and ovulation promotion are not pregnant; 2. Second generation IVF is called ICSI, also called intracytoplasmic single sperm injection, which is suitable for men with particularly poor sperm and low fertilization rate. If there is no sperm in the semen, testicular penetration ICSI can be done; 3. Third generation IVF is mainly for people with single gene genetic disease, before doing IVF both spouses have single gene genetic disease, after doing IVF embryos are formed, a few cells are drawn from the embryo for examination to see if the gene is the same as the disease, if the same embryo needs to be cancelled, called pre-implantation embryo genetic diagnosis.