Long protocol IVF men should be ovulated on the day of the woman’s egg retrieval and then spermatozoa should be retrieved. Spermulation for men doing IVF is an extremely important process in the IVF process. The IVF men’s spermatogenesis and the women’s IVF egg retrieval will be assigned to take place on the same day, i.e. the men will start spermatogenesis after the women’s eggs are retrieved. Prior to sperm expulsion, it is important to prevent over-exertion, sauna bathing, excessive alcohol and tobacco use, and taking medications that can affect the male sperm. It is recommended to stop sperm retrieval if you have recent fever symptoms with body temperature over 37.5℃. If special circumstances of mental stress can not be in the hospital spermatozoa, need to go to the hospital outside the spermatozoa, semen should be sent to the hospital outpatient test in 30 to 60 minutes, cold days pay attention to warmth, the temperature to close to the human body temperature is appropriate.