Unopened insulin needs to be refrigerated, and is usually kept at 2-8°C, the freezer level of the refrigerator is sufficient, and when placing it, be careful to keep it at a distance from the inside wall of the refrigerator to prevent the insulin from freezing. Before use, insulin should be placed at room temperature for a period of time, so that the temperature of the insulin is close to room temperature, and then only for subcutaneous injection to prevent skin irritation, note that insulin should never be frozen. Because insulin is a small molecule of protein, freezing will cause insulin to produce denaturation loss of efficacy, if the insulin has been opened and placed directly at room temperature can be stored, but must be placed in a cool, dry place, to avoid humid environments, direct sunlight, after the opening of insulin should not be more than 1 month of use.