Genital herpes is is caused by having the herpes simplex virus type 1 or type 2. Typical genital herpes is not difficult to diagnose. If the clinical manifestations are not obvious and typical, laboratory tests are needed, which include cytological examination, virus isolation and identification, serological methods and antigen detection methods. The common laboratory tests for genital herpes are as follows: a. Cytological examination: picking vesicles or scraping a small amount of lesion from the ulcerated surface at the base of the herpes for smear and looking for large multinucleated giant cells and eosinophilic inclusions in the nucleus of multinucleated giant cells by Wright-Giemsa staining or Papanicolaou staining. However, this method cannot distinguish between HSV infection or related herpesvirus-like diseases such as varicella-zoster virus infection, and may yield positive results only when blisters are present in the acute phase. It is generally used as a primary screening test. Immunocytochemical examination: usually a smear of skin lesion cells is used, which is specially treated and observed under a fluorescent microscope to detect antigens; genital herpes virus-infected cells are seen to fluoresce bright green. Virus isolation: Usually a specimen is obtained from the bottom of the blister within 1-3 days of onset with a cotton swab, and the virus is isolated and cultured using human embryonic fibroblasts, human amniotic cells, and kidney cells, and then identified and confirmed by immunofluorescence. The prerequisite for successful isolation is accurate sampling and inoculation as soon as possible, but because of its complicated operation, high cost and high technical requirements, it is not yet commonly used. Fourth, the antibody test method: HSV antibody test can not be used as the basis for the diagnosis of genital herpes. However, cases, such as determining whether HSV has been infected before; infected persons with atypical symptoms; assessing the risk of infection in newborns born to spouses of patients with genital herpes and the success rate that may be obtained when vaccinating against herpes simplex. This is done by applying an enzyme-linked immunosorbent assay (ELISA) to detect HSV-1 and HSV-2 IgM/IgG antibodies in the serum. V. Gene detection method: HSV-II-DNA of skin lesions is detected by PCR fluorescence quantification. it can directly detect HSV-2 pathogen at the damage site in patients with genital ulcerative diseases with high sensitivity and specificity, which greatly improves the ability to confirm the diagnosis of HSV in patients with genital ulcers. It is more expensive and requires higher operational techniques, laboratory conditions and equipment. These methods require high laboratory conditions and techniques for laboratory diagnosis, and can be performed only in laboratories with good equipment conditions. The choice of the test method in those cases and the interpretation of the conclusions reached must be subject to the opinion of a professional physician.