It is not necessary to use morning urine for a pregnancy test, but you should try to use morning urine. This is because at night, when people are less active and urinate less, the hormone content in the urine is higher after a long period of storage, which makes it more accurate to detect a woman’s pregnancy. To ensure a reasonable method of testing whether you are pregnant, urine must be kept in the bladder for more than 4 hours to ensure the accuracy of the test, so it is more accurate to test in the morning than at other times. Women can use morning urine to test for pregnancy about 7-10 days after having intercourse, or they can go to the hospital to have a blood test.