The accuracy of the afternoon test depends on the method of detection, and also depends on the time of pregnancy. With blood tests, both morning and afternoon tests are more accurate, but urine tests are not necessarily, because the concentration of the first urine in the morning is higher, and after the afternoon the hormone concentration is lower. If a woman has been pregnant for a long time, for example, she has stopped menstruating for 40 days, the hormone level in her body is high at this time, and the urine test is accurate both in the morning and in the afternoon. If it’s only been 30 days since menopause, it’s best to pee for the first time in the morning, because morning pee is more accurate.