Embryo thawing and resuscitation usually takes about 2 to 4 hours, embryo thawing usually takes about half an hour, and thawed embryos are usually recommended to be incubated in a culture solution for at least 2 to 4 hours. Thawing embryos means that the laboratory removes the frozen embryos from the liquid nitrogen tanks and places them in a number of resuscitation solutions for 2 to 3 minutes at a time, which usually takes about half an hour to thaw. After thawing the embryos also need to be rinsed and cultured in an incubator waiting for the blastocyst and blastocyst cavity to expand concentrically. The thawed embryo is considered to be successfully resuscitated if the number of surviving cells is greater than or equal to 50% and the blastocyst cavity is reexpanded within 2 to 4 hours, based on the observation of the recorded embryo growth. Generally, thawing and resuscitation of embryos is performed on the day of transfer after it has been determined that there are no abnormalities in the mother’s uterine lining. If the thawing and resuscitation of the embryo fails within 2 to 4 hours, the transfer cannot take place.