The reference range of blood amylase is about 35~135 U/L, if it is higher than the reference range, it is recommended to consult a doctor for diagnosis and treatment. High amylase is mainly seen in acute pancreatitis, but also in pancreatic cancer, cholecystitis, cholangitis, pancreatic duct or bile duct obstruction, mumps, peritonitis, acute appendicitis, renal failure or renal insufficiency, intestinal obstruction, perforated ulcers, and ruptured tubal ectopic pregnancies, etc., but the magnitude of serum amylase elevation in these diseases is generally small. Elevated serum amylase, one of the important diagnostic indicators of acute pancreatitis, begins to rise in activity 2 to 12h after the onset of the disease, peaks in 12 to 72h, and returns to normal after 3 to 4 days. Although the degree of elevated amylase activity is not necessarily related to the degree of pancreatic injury, the greater the degree of elevation, the greater the likelihood of acute pancreatitis, so amylase is used as the first choice for the diagnosis of acute pancreatitis. The diagnosis of pancreatitis can be confirmed by two out of three of the above three criteria: persistent abdominal pain, amylase that is more than three times higher than normal, and peripancreatic exudate on imaging. When acute pancreatitis is suspected, the patient’s serum and urine amylase activity should be observed continuously and dynamically, and can also be analyzed together with clinical conditions and other tests, such as pancreatic lipase and trypsin, to make a diagnosis. If there is any discomfort or abnormality in the indexes, it is recommended to consult a doctor in time for diagnosis and treatment.