Any virus will survive on an object, but the length of time it survives varies, and hepatitis B virus is no exception. The hepatitis B virus requires harsh conditions to survive in vitro, and the invasive power of the hepatitis B virus is very weak, so it must often enter the blood directly before infection occurs. However, there are exceptions, when the hepatitis B patients applied puncture needle accidentally, dental repair, dental scaling repeatedly applied medical equipment, tattoo, eyebrow tattoo, eyeliner repeatedly applied needle, if not thoroughly disinfected after use, very easy to occur hepatitis B virus transmission, so in order to avoid the occurrence of hepatitis B virus transmission, clinical often contact puncture needle medical personnel, should be vaccinated against hepatitis B vaccine. Repair teeth, scaling to formal medical institutions, tattoos, eyebrow tattoo, eyeliner should also go to formal business premises, it is best to use disposable needles, or vaccination against hepatitis B before the implementation of needling.