E. coli detection methods are as follows: First, routine stool tests plus fecal occult blood tests, if the test results suggest a bacterial infection, then it is necessary to improve the stool culture plus drug sensitivity test to further clarify the type of infecting bacteria and the type of sensitive antibiotics, which can be used as a monitoring method of E. coli. Second, the anal or rectal secretion test, can also be used as a monitoring means of E. coli bacteria. Third, E. coli can also be found in other parts of the body, such as the oral cavity, stomach, respiratory tract, etc. If in the oral cavity, an intraoral secretion test can be performed to detect E. coli infection, and if in the stomach, a case tissue biopsy after gastroscopy may detect E. coli. If it is in the respiratory tract, sputum culture plus drug sensitivity test can be performed to detect E. coli. In short, E. coli detection is usually found by specimen assay.