In vitro fertilization and embryo transfer is a technique that has flourished for more than three decades. It is a technique in which a couple’s eggs and sperm are fertilized outside the body, developed into embryos and transferred into the uterine cavity, also known as IVF. With the birth of Louis Brown in 1978, IVF gained worldwide attention. With the booming of assisted reproductive technology, more elements have been developed based on in vitro fertilization and embryo transfer, including microfertilization, embryo freezing and pre-implantation genetic diagnosis. Indications (1) Tubal infertility: Tubal factor is one of the common factors of infertility and one of the main indications for IVF-ET treatment. Infertility is caused by inflammation of the fallopian tubes, peri-tubal lesions, mechanical causes caused by tubal pregnancy or post-ligation that prevent the transport of sperm, the uptake of eggs, the fertilization of eggs and sperm and the transport of fertilized eggs to the uterine cavity, or by cytokines produced by fluid in the fallopian tubes that affect sperm-egg treatment, the fertilization environment and embryo development. Patients with tubal infertility may opt for surgical treatment or IVF-ET treatment. IVF-ET offers a chance of pregnancy, especially for patients who are still infertile after bilateral tubal obstruction, severe pelvic adhesions, tubal plastic surgery or recanalization. However, it should be noted that fluid in the fallopian tubes may affect the endometrial tolerance and have toxic effects on the embryo, thus reducing the success rate of IVF-ET. (2) Male factor: Patients who have not conceived due to multiple intrauterine inseminations with few, weak or deformed sperm can be treated with IVF-ET. Or due to severe oligozoospermia or obstructive azoospermia, single sperm intracytoplasmic microinjection technique can be used, which offers the possibility of fertility in men with infertility that cannot be resolved by traditional methods. (3) Endometriosis: Severe endometriosis that has not been treated with medication or surgery can be helped by IVF-ET technology. Severe endometriosis affects the ovarian tissue structure, which may increase the dose of superovulatory gonadotropins and affect both the number of eggs obtained and the quality of the eggs. (4) Those with polycystic ovary syndrome who have failed to respond to treatment: those with ovulation disorders and intractable polycystic ovary syndrome who have failed to conceive after multiple ovulation treatments and intrauterine insemination may opt for IVF-ET treatment. (5) Unexplained infertility and immune infertility: Patients with unexplained infertility and immune infertility who have failed to conceive after other treatments, especially those who have failed to conceive with intrauterine insemination, can choose IVF-ET treatment. There is a possibility of finding egg quality problems or fertilization disorders during the treatment. Currently, there is no waiting list for IVF at our center, so bring your previous test report to the clinic and both couples will be seen together.