When performing Down’s syndrome screening, it is important to note that: 1) the timing of the Down’s syndrome screening should be mastered, generally between 16 and 18 weeks; 2) the results of the Down’s syndrome screening are affected by the age, weight, height and other factors of the pregnant woman. When conducting Down’s syndrome screening, it is important to provide accurate information; 3. Before Down’s syndrome screening, it is generally not necessary to hold urine or fasting; 4. Pregnant women who smoke or have a history of gestational diabetes will affect the accuracy of the screening results; 5. Before conducting Down’s syndrome screening, pregnant women should pay attention to adequate rest, do not strain, pay attention to maintain a happy mood and avoid excessive emotional fluctuations; 6. The results of Down’s syndrome screening, such as abnormal screening results, should be further examined.