Non-invasive paternity testing is a test method that involves taking peripheral blood from a pregnant woman’s vein, amplifying the fetal DNA from the peripheral blood, and then determining paternity through DNA testing, which has certain limitations. Non-invasive paternity testing is the opposite of invasive paternity testing. In the past, paternity testing was mostly performed by amniotic fluid extraction because the amniotic fluid contains fetal DNA, and DNA testing is used to determine paternity, but amniocentesis is invasive and risky. The DNA test is less invasive because it is performed by simply drawing blood from the peripheral blood of the pregnant woman, which is why it is also known as non-invasive paternity testing. This is the result of scientific and technological developments, and recent scientific research has revealed that a small amount of fetal DNA can enter the mother’s body and can be extracted for DNA amplification, enabling the completion of non-invasive paternity testing.