Acne, which is generally referred to as acne in medical terms, requires examination of microorganisms, fungi, endocrine hormones and other items. 1. Microbiological examination: It is mainly used to determine whether acne is due to microorganisms such as mites, so as to exclude the presence of mite infection. 2. Fungal smear test: mainly for patients with a single pattern of acne lesions on the trunk or acne vulgaris, to determine whether it is caused by fungi such as Malassezia. 3. Endocrine hormone examination: androgens play a great role in the pathogenesis of acne, if there is severe acne on the face, accompanied by irregular menstruation, endocrine hormone examination can be conducted. It is recommended that acne patients go to the hospital for relevant examinations and standardized treatment under the guidance of professional doctors.