1, indications: (1) in vitro fertilization – embryo transfer indications: ① female side of various factors caused by gamete transport obstacles; ② ovulation disorders; ③ endometriosis; Shandong University of Traditional Chinese Medicine, the Second Affiliated Hospital Center for Reproductive Medicine Cui Wei ④ male side of oligo, weak spermatids; ⑤ unexplained infertility; ⑥ immune infertility. (2) indications for intracytoplasmic monosperm microinjection: ① severe oligozoospermia, weak spermatozoa, abnormal spermatozoa; ② irreversible obstructive azoospermia; ③ dysfunction of spermatogenesis (exclude those caused by genetic defects); ④ immune infertility; ⑤ failure of in vitro fertilization; ⑥ spermatozoa acrosomal abnormality; (7) need to carry out preimplantation genetic examination of the embryo. (3) Indications for preimplantation embryo genetic diagnosis Currently, it is mainly used for single gene-related genetic diseases, chromosomal diseases, sex-linked genetic diseases and high-risk groups that may give birth to abnormal children. 2, contraindications: (1) one of the following conditions, in vitro fertilization – embryo transfer and its derivatives ① either man or woman suffering from serious mental disorders, acute infections of the genitourinary system, sexually transmitted diseases; ② suffering from the “Maternal and Child Health Law” stipulates that should not give birth, currently unable to pre-implantation genetic diagnosis of genetic disorders; ③ either party (iii) Any one of them has serious bad habits such as drug addiction; (iv) Any one of them has been exposed to teratogenic amount of rays, poisons, medicines and is in the period of action. (2) The uterus of the female partner does not have the function of pregnancy or serious physical disease cannot bear pregnancy.