To make a diagnosis of hepatitis B minor triplet, a two-to-one quantification test is required. The two-to-one quantification includes five tests, including positive hepatitis B surface antigen, positive hepatitis B E antibody, and positive hepatitis B core antibody, which refer to the small triplet of hepatitis B. After the diagnosis of hepatitis B minor tri-positive is made, it is necessary to determine what period the minor tri-positive is in, whether it is in hepatitis B carrier status or chronic hepatitis B or cirrhosis. Further improvement of liver function, quantitative measurement of hepatitis B virus, imaging of the liver, and fetoprotein tests are required.