The exact figure for the accuracy of tracheoscopic puncture biopsy varies from one disease diagnosis to another, from one operator to another, etc. However, its diagnostic positivity is very high and it is a very important method of pathologic diagnosis. Tracheoscopic puncture biopsy is based on the site of the lesion shown on imaging, combined with what is seen on the fiberoptic scope. The front end of the fiberoptic scope is aligned with the opening of the selected bronchus, and the catheter is twisted and pumped under fluoroscopy and the patient is rotated appropriately, so that the head end of the catheter is inserted as far as possible into the diseased bronchus to be examined, and observed from multiple angles in order to obtain diagnostic information of selective bronchography in the lesion area. Such a close observation and sampling, combined with imaging localization, has a high success rate. According to the data in the literature, the positive rate of puncture sampling 97. 3% ( 110 /113); biopsy results compared with postoperative follow-up pathology results or surgical results, the correct rate of histological diagnosis 95. 6%, and according to the data, tracheoscopic puncture biopsy complications are fewer and lighter than other puncture biopsy. It is important to communicate with the doctor about the treatment and diagnosis plan before doing the examination to avoid delaying the condition.