Both testosterone and dihydrotestosterone belong to the range of gonadal hormones, which can promote the development of male sexual organs and maintain male secondary sexual characteristics, and have important roles. The differences between testosterone and dihydrotestosterone are as follows: 1. Testosterone is the main androgen in the body, and all male testosterone is synthesized in the testicular mesenchymal cells. dihydrotestosterone is a steroid hormone, which is produced by testosterone under the action of 5-alpha reductase, so dihydrotestosterone is the metabolite of testosterone. 2. Testosterone plays an important role in the differentiation and development of male reproductive organs, as well as the maintenance of male secondary sexual characteristics. Dihydrotestosterone plays an important role in the proliferation, development and secretion of the prostate. 3. Testosterone and dihydrotestosterone work together to maintain normal male reproductive function. Certain diseases, such as 5-alpha reductase deficiency, can lead to a decrease in dihydrotestosterone and abnormalities in sexual differentiation, even though they do not affect the secretion of testosterone.