The measurement of serum vitamins is of great clinical significance because vitamins are trace organisms essential to human growth and metabolism, and a deficiency of vitamins can cause a range of physiological dysfunctions and pathologic changes. Serum vitamin measurements include vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin K and folic acid in the body. The human body cannot carry out normal physiological metabolism in the absence of vitamins, for example, the three major nutrients protein, fat and sugar must be transported by vitamins before they can be fully absorbed and digested by the body. Therefore, the determination of vitamin deficiency or reduction in the body, can play a certain role in the treatment and prevention of the effect, in addition to serum vitamin assay can also guide a certain amount of children rickets, cretinism and other diseases for symptomatic treatment.