The diagnosis of hemolytic anemia is based on clinical signs combined with laboratory tests. There may be pallor, splenomegaly, elevated unconjugated bilirubin in blood, etc. 1. Clinical manifestations: acute hemolytic anemia may manifest as aching limbs, followed by high fever, pallor, hemoglobinuria, jaundice, and in severe cases, acute renal failure. Chronic hemolytic anemia manifests as anemia, splenomegaly, liver function impairment, long bone deformation and so on. 2. Laboratory tests: for the increased destruction of red blood cells, there are: blood unconjugated bilirubin, urobilinogen, plasma free hemoglobin is elevated, urine hemoglobin, urine iron hemosiderin are positive. Tests for compensatory red lineage hyperplasia include an elevated reticulocyte count, nucleated red blood cells on peripheral blood smear, and a bone marrow examination that reveals exuberant red lineage hyperplasia. If the diagnosis of hemolytic anemia is confirmed, early and standardized treatment is recommended to reduce the adverse effects of the disease.