1.What tests are needed before implementing assisted reproduction technology? (1) Female partner must check: sex hormone six items, blood routine + blood type two items, liver and kidney function, coagulation function, teratogenic eight items, hepatitis B half + hepatitis C + HIV + syphilis, anti-sperm antibodies, anti-cardiolipin antibodies, urine routine, cervical cell smear, white belt routine + chlamydia + gonococcus, gynecological ultrasound examination, electrocardiogram, chest X-ray. (2) Optional items for the female partner: chromosome, post-coital test, closed antibody, gene test for geodystrophy. (3) Mandatory items for male partner: semen routine + morphological analysis + sperm tail hypotonic swelling test + eosin staining + sperm membrane antibody, blood routine + blood type two, blood anti-sperm antibody, hepatitis B two-to-one + hepatitis C + HIV + syphilis, semen mycoplasma + chlamydia + gonococcus, semen leukocyte peroxidase staining examination. (4) The male side optional check items: chromosome + Y chromosome microdeletion, sex hormone, testicular biopsy, testicular ultrasound examination, sperm acrosome reaction, prostate fluid routine, urine routine. 2.What documents are required to do artificial assisted technology? According to the relevant policies of the Ministry of Health, couples who undergo assisted reproductive technology must provide the hospital with both parties’ ID cards, marriage certificates, family planning certificates (family planning service certificates, second birth certificates, birth certificates issued by family planning offices at town level or above), or if the couple is a foreigner (including Hong Kong, Macau and Taiwan), only the marriage certificate, both parties’ ID cards or passports are required. All documents need to be photocopied for record. The family planning certificate is valid for one year, if your certificate has expired, please visit the relevant department for annual review. 3.What does intrauterine insemination (IUI) mean? The semen of the husband is injected into the uterine cavity during ovulation of the woman after washing and screening for optimization. It is suitable for patients with husband’s impotence, premature ejaculation, lack of cervical mucus during female’s ovulation and other cervical factors of infertility, partial immune infertility, slight oligospermia and weak sperm of male partner, etc. 4.What is conventional IVF-ET? IVF-ET is the abbreviation of in vitro fertilization-embryo transfer, which is the process of removing the eggs and sperm of infertile couples, fertilizing them in vitro, culturing them into embryos and then transferring them into the patient’s uterine cavity for implantation to achieve pregnancy, commonly known as IVF. 5. What are the conditions suitable for IVF? (1) congenital occlusion or abnormal development of the fallopian tubes, such as atresia of the umbilical end into a blind end; (2) destruction or obstruction of the fallopian tubes (e.g. after pelvic inflammatory disease), loss of egg collection function and ineffective treatment; (3) removal of the fallopian tubes after ectopic pregnancy, or infertility of the fallopian tubes despite conservative treatment; (4) inability to conceive after surgery and medication for endometriosis; (5) (6) patients who have been married for many years and have unexplained infertility who have failed to conceive after repeated artificial insemination and are too old to conceive; (7) patients with low sperm count or poor viability; (8) patients with azoospermia on routine semen examination but mature sperm on testicular biopsy; (9) patients with hereditary diseases such as hemophilia, pseudohypertrophic myotonic dystrophy, chromosomal abnormalities such as congenital fetus (9) Those with genetic disorders such as: hemophilia, pseudohypertrophic muscular dystrophy, chromosomal abnormalities such as congenital dullness, single gene disorders such as sickle cell anemia and thalassemia. 6. How long does it take to determine the duration of IVF treatment? The whole process takes about 2-3 menstrual cycles. The normal pre-IVF examination is 2 weeks, blood will be drawn on the 2nd-4th day of menstruation, and after the menstruation is clean, chlamydia and cervical smear will be performed, and all the information and “three certificates” will be brought to build the medical record. On day 20-23 of menstruation, we will start IVF-ET treatment, applying GnRHa (down regulation injection), 1 week after down regulation, we will check ultrasound to see if there are any cysts, 14-18 days after down regulation, we will start to watch ovulation injection, ultrasound to monitor follicles, routine ovulation time is about 9-12 days, when follicles are big enough, we will inject HCG (around 9 pm), 35-36 hours after HCG injection: female partner The female partner will retrieve the eggs and the male partner will retrieve the sperm and return to the hospital 3 or 5 days later for embryo transfer.