Immunohistochemistry and immunofluorescence are both immunogenic and both are similar in principle, differing only in the chromogenic agent. Immunohistochemistry uses enzymes for color development, whereas immunofluorescence generally does not use enzymes for color development. In addition, immunohistochemistry is usually viewed in the bright field, while immunofluorescence is viewed in the dark field. Patients need a clear diagnosis of the disease first, and without an accurate diagnosis, there is no accurate treatment. The real purpose of both immunohistochemistry and immunofluorescence is to clarify the pathological diagnosis so that the patient can receive precise treatment.