The new crown nucleic acid testing practices include nasopharyngeal swab, oropharyngeal swab, anal swab, and deep cough sputum. 1. Nasopharyngeal swab: the sampling personnel gently support the head of the person to be collected, one hand swab sticking to the nostrils into the bottom of the lower nasal tract along the back of the depth slowly, to be swabbed to the top of the nasopharyngeal cavity to reach the back wall of the swab, gently rotate for a week, and then slowly take out the swab. 2. Oropharyngeal swab: the person to be collected first rinses the mouth with saline, the sampling personnel will put the swab into the sterile saline to moisten, the person to be collected tilts the head slightly, the mouth opens wide, and sends out the “ah” sound, reveals the two sides of the pharyngeal tonsils, the swab will cross the root of the tongue, in the person to be collected in the two sides of the pharyngeal tonsils a little bit of forceful back and forth to wipe at least 3 times, and then in the posterior pharyngeal wall up and down to wipe the swab. Swab the posterior pharyngeal wall up and down at least 3 times. 3. Anal swab: the patient takes the knee-chest position or side-lying position, with a disinfected cotton swab gently inserted into the anus 3 cm to 5 cm, and then gently rotate and pull out, and immediately put it into the 15 ml external screw-cap sampling tube containing 3 ml to 5 ml virus preservation solution, discard the tail, screw the cap tightly, and promptly send it to be examined. 4. Deep coughing of sputum: After asking the patient to cough deeply, collect the coughed-up sputum in a 50 ml screw-cap plastic tube containing 3 ml of sampling solution.