There are many precautions for urine bacterial culture, as follows: 1, specimen retention phase, it is recommended that the patient must wash the vulva before the specimen is retained, and must be retained in the middle of the urine, to avoid miscellaneous bacteria mixed into the urine; 2, with a clean container to hold the urine, if the container is not clean will also lead to urine contamination; 3, after the retention of the specimen must be sent in a timely manner, generally recommended within 2 hours, because the time is too long If the specimen is left at room temperature, it will lead to the growth of miscellaneous bacteria; 4, it is recommended that clinical doctors generally send the test for 3 consecutive days; 5, urine bacterial culture should be left before the application of antibiotics, and do drug sensitivity test, if the application of antibiotics and then do urine bacterial culture, it will lead to false negative results.