Biopsy, referred to as biopsy, uses forceps, excision, fine needle puncture, scratching and other methods to remove diseased tissue from living tissue. Widely used in clinical practice, biopsy can be carried out in the following cases: 1) unable to specify the cause of the disease; 2) assessing the effect of treatment, such as evaluating the effectiveness of preoperative targeted therapy and neoadjuvant chemotherapy for breast cancer; 3) unable to specify the inflammation and tumor; 4) unable to specify the type of inflammation, acute inflammation, chronic inflammation, granulomatous inflammation, fungal infection, or parasite infection; 5) unable to specify the nature of the tumor, such as benign tumors, malignant tumors 6. Tumor typing was performed.