The six sex hormone tests are mandatory for female endocrine examinations to understand the basic functions of the ovaries and to diagnose reproductive and endocrine diseases. During the normal menstrual cycle, each of the six sex hormones has its own secretion curve, and different test results will appear at different times of the test, and a comprehensive analysis of the six hormones is required to make a diagnosis. The timing of the test is particularly important, and is usually chosen on an empty stomach in the morning of the second to fifth day of menstruation. The six sex hormone tests include the pituitary gonadotropins: follicle stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL), as well as estrogen, progesterone, and androgen produced by the ovaries. The levels of these six hormones in the serum are tested to understand the basic function of the ovaries and to diagnose related diseases. The best time for the test is 2 to 5 days after the menstrual period, which is the early follicular period and can reflect the functional status of the ovaries. However, for patients with amenorrhea, which is a special case, the test can be performed at any time. Also note that you should not apply sex hormone drugs, including progesterone, estrogen, and birth control pills, for at least one month before having the test, as this may affect the results. Take a break before the blood draw to avoid hormonal changes caused by exercise. The sex hormone six test has strict requirements on the time of the test. In order to get accurate results to assist in diagnosis, it is recommended that the test be performed at the proper time as requested by the doctor to ensure the accuracy of the test results.