The normal range for total anticardiolipin antibodies is negative by enzyme-linked immunosorbent assay (ELISA) and positive when P/N ≥ 2.1. Anti-cardiolipin antibodies are autoantibodies against various negatively charged phospholipids in the body. This antibody can bind to phospholipids on endothelial cells or platelet membranes, disrupting cellular function, creating a hypercoagulable state of the blood, and also causing hemolytic anemia. Anti-cardiolipin antibodies can be detected clinically by enzyme-linked immunosorbent assay (ELISA) testing, which is negative in the normal range and positive when P/N ≥ 2.1. A positive test helps to diagnose rheumatoid arthritis, spontaneous abortion, and intrauterine stillbirth. Anti-cardiolipin antibody test, commonly used in the diagnosis and differential diagnosis of immune diseases, can also be used as a prospective indicator of spontaneous abortion, it is recommended to seek medical attention and standardize the treatment under the guidance of professional doctors.