The most common symptoms of Candida infection are leucorrhea, burning and itching of the vulva and vagina, difficulty in urination due to external factors, and erythema vulvae (mycosis or Candida vulvovaginitis), which is typically curd-like or flaky, with a highly red and swollen vaginal mucosa and white thrush-like patches that can be easily peeled off, with a vesicular substrate of damaged mucosa or shallow ulcers that can leave petechiae in severe cases. The leucorrhoea does not always have these typical features, but can range from watery to curd-like leucorrhoea. Some are completely thin and clear plasma exudate, which often contains white flakes. The itching symptoms of mycosis vaginalis during pregnancy are particularly severe and even restless, painful, and can also have symptoms such as urinary frequency and painful intercourse. In addition, there are about 10% of women and 30% of pregnant women who are carriers of mycobacteria but do not have any clinical manifestations. There is usually no difficulty in diagnosing mycotic vaginitis based on the typical clinical manifestations and visual examination of vaginal excretions. However, in atypical cases, in order to confirm the diagnosis, anyone suspected of carrying mycobacteria, or to understand the effect of treatment must be examined by vaginal discharge. Vaginal discharge can be taken directly onto a slide, add a small drop of isotonic sodium chloride solution or 10%-20% potassium hydroxide solution with a coverslip, microscopic examination by microheating, red and white blood cells and epithelial cells immediately dissolve, while mycobacteria that is in the form of thread-like fibers or mycelia (mycelia), and microspores or molecular spores (conidia) attached but this method is less reliable (60%). If a smear of vaginal secretion is taken by Gram staining, microscopic clusters of Gram-positive intensely stained ovoid spores can be found or pseudomycorrhizal hyphae can be seen connected to the germinating cells in chains or branches, which are easily recognized and the reliability can be increased to 80%. In addition, we should also pay attention to the relevant causative factors such as the application of high doses of steroids or broad-spectrum antibiotics, and diabetic patients need to do urine glucose and blood sugar tests.