Blood sampling for liver function tests after eating may lead to abnormal fluctuations in aminotransferases and elevated total bilirubin in the blood, which may result in biased liver function test results. Liver function tests generally require fasting for 8-12 hours, as eating may affect the correctness of liver function test results. Since the liver function test program includes transaminases, bilirubin, albumin, alkaline phosphatase and other indexes, the body will absorb the protein in the food after a meal, which will cause the protease to rise, and then cause abnormal fluctuations in transaminases, so the correctness of the liver function test results may be affected after eating. In addition, after eating, the gallbladder will secrete bile, which will be absorbed together with the digested food, causing the total bilirubin in the blood to rise, and also affecting the correctness of the results of liver function tests. Therefore, it is important to follow the doctor’s instructions and fasting when taking blood for liver function test in order to ensure the correctness of the results.