Myocardial enzyme profile mainly includes five items: alanine aminotransferase, glutamate aminotransferase, phosphocreatine kinase, phosphocreatine kinase isoenzyme, and lactate dehydrogenase. Cardiac enzyme profile is mainly used to check the test markers of myocardial injury and necrosis, in which phosphocreatine kinase isoenzyme is the best cardiac enzyme for diagnosing acute myocardial infarction, when infarction occurs, the enzyme can be as high as 10-15 times of the normal value, with a high specificity, and its elevated level can reflect the area of necrosis of the myocardium. Lactate dehydrogenase, alanine aminotransferase, glutamine aminotransferase are widely present in all tissues of the human body, and phosphocreatine kinase is mainly distributed in the cytoplasm and mitochondria, which are not specific for the diagnosis of myocardial injury and necrosis, and can be used as a reference. In addition to the myocardial enzyme spectrum, the clinical sensitivity of troponin for the diagnosis of acute myocardial infarction is also very high, and the change of myoglobin and other indicators can also be used as a reference indicator of myocardial injury. The diagnosis of myocardial injury and necrosis such as acute myocardial infarction or myocarditis can not only rely on myocardial enzyme profiles and other laboratory tests, but also combined with clinical symptoms and electrocardiogram and other auxiliary tests to make a comprehensive judgment.