Homocysteine is a metabolic product of the human body, which is not present in the blood under normal conditions, but if it is present in excess it can lead to atherosclerosis, which can cause cardiovascular and cerebrovascular diseases, and is also an indicator reflecting the health of the human body. There are two methods for the determination of blood homocysteine, firstly, the isotope method is determined by ¹⁴ C. The results measured by this method are very specific, but because it is a radioactive substance with a certain degree of contamination, the scope of promotion is not very large, and it is mainly determined by immunological methods. The specific operation is that the vast majority of homocysteine in normal human plasma exists in the form of bound albumin, so it cannot be detected directly. A small percentage is present in the form of compounds, which can be directly detected by using special detection reagents and homocysteine is isolated, and this method is simple and easy to popularize.