Hemolytic disease of the newborn is mainly due to the mother and baby’s blood type incompatibility, the mother’s blood type antibody through the placenta caused by the fetus, the newborn’s red blood cell destruction, resulting in the homozygous immune hemolysis. Neonatal hemolytic disease mainly includes ABO hemolysis and RH hemolysis, of which ABO hemolysis is the most common, if the mother is type O blood, the father is type A or B blood, then the baby may be type A or B blood, it is easy to appear neonatal ABO hemolysis. Clinically, the main manifestations are jaundice, anemia, liver and spleen enlargement. Treatment mainly includes light and medication, which includes intravenous albumin, etc. If the baby’s jaundice is severe and reaches the indication of blood exchange, it is even necessary to be treated with blood exchange. Therefore, if the jaundice appears within 24 hours after the birth of the newborn, the jaundice progresses quickly, the mother is type O blood, the baby may be type A or B blood, considering that there may be the possibility of ABO hemolysis, it is necessary to go to the pediatric outpatient clinic of the local hospital in a timely manner, to do the identification of the blood group and the test of anti-human globulin and other related tests to determine whether the blood is hemolytic, and in addition to liver function, blood routine tests to determine the severity of the hemolytic jaundice of the baby, and consider further treatment to reduce the yellowing. In addition, liver function and blood test should also be performed to determine the severity of hemolytic jaundice, and further treatment should be considered.