WHO recommends that a couple who have lived together for more than one year after marriage, have a normal sex life, and have not taken any contraceptive measures, and whose female partner is infertile due to the male partner, is called male infertility. Diagnosis of male infertility 1. Male physical examination (performed by male physicians) To understand male sexual characteristics and whether there are abnormalities in the development of testes, epididymis, spermatic veins and vas deferens. To obtain basic information on male reproductive status. 2.Semen analysis To understand semen volume, semen color, PH value, sperm concentration, vitality, activity rate, survival rate, malformation rate, effective sperm count, liquefaction status, etc., and to assess male fertility. 3.Scrotal ultrasound Check the volume of testicles, nodules of epididymis, varicose veins and reflux of spermatic veins, and the presence of syringomyelia to evaluate male reproductive organs from the morphological point of view. 4.Microorganisms of the reproductive system Check mycoplasma, chlamydia, eugenics IV, HIV, syphilis spirochetes, hepatitis B virus, etc. to exclude common pathogenic microorganisms that can cause reproductive system infections. 5.Anti-seminal membrane antibodies Anti-seminal membrane antibodies, namely MAR, are recommended by WHO to screen for the causes of male immune infertility. 6.Seminal plasma biochemistry To understand the seminal plasma PH value, white blood cells, acid phosphatase, zinc, fructose, α-glucosidase, etc., to assess the secretory function of male accessory gonads and the degree of patency of the vas deferens. 7.Seminal plasma elastase The reliability of seminal plasma elastase as a diagnostic and healing test for occult reproductive tract infection has been recognized by WHO. 8.Sperm function Sperm function determination, which more objectively reflects the fertilization ability of sperm, is a necessary supplement to the routine semen examination. The acrosome enzyme assay, which mainly looks at the ability of sperm to cross the zona pellucida. 9.Sperm DNA fragmentation rate This test reflects the degree of sperm DNA damage, and is a common indicator for judging idiopathic male infertility, recurrent miscarriage, assisted reproduction and eugenic prognosis. 10.Post-coital test This test can be used to know whether the sperm can penetrate the cervical mucus and whether it has a good fertilization ability. The best condition for the test is not smoking, not drinking, not overworking, no cold and fever, no major diseases in the past 2~3 months, and the 3rd~5th day after semen emission (including intercourse, seminal emission, masturbation, etc.), not more than 7 days, to the professional sperm collection room of regular hospital reproduction department to keep the semen specimen, avoid spilling semen and ensure the integrity of the specimen.