If the disease is to be diagnosed accurately and in a timely manner, the appropriate diagnostic tools also need to be selected. For most solid lesions, obtaining the lesion tissue for histological examination remains the most accurate method. Why is a biopsy necessary in diagnosing disease? This is because, for many difficult cases, the diagnosis is still difficult even through signs and symptoms, laboratory tests, and imaging, which requires biopsy of the lesion specimen to confirm the disease directly and quickly from a cellular, histological, microbiological, and even molecular perspective. The current methods of biopsy are: surgical biopsy, X-ray guided biopsy, ultrasound guided biopsy, CT and CT fluoroscopy guided biopsy. The safest and most accurate method is recognized as CT and CT fluoroscopy guided biopsy. Less invasive and more accurate, CT fluoroscopy combines the advantages of real-time ultrasound imaging with the clarity of CT images, while avoiding the disadvantages of invasive and costly surgical biopsies, providing great convenience for biopsies. Using CT fluoroscopy, we can perform accurate puncture biopsies of lung lesions over 1 cm in diameter. For solid lesions, its diagnostic accuracy can reach more than 95%. It is suitable for the diagnosis of a variety of difficult diseases. CT and CT fluoroscopy-guided biopsy can be used in the following areas: (1) benign and malignant tumors to be confirmed. (2) Malignant tumors that are suspected to be non-surgical, but need to be clarified in terms of cell type in order to determine treatment options. (3) Metastatic tumors to be confirmed. (4) Diseases that cannot be clearly diagnosed by other tests. (5) Follow-up after tumor treatment to assess whether the tumor has recurred or residual. In general, CT and CT fluoroscopy guided biopsy is one of the most important tools for diagnosis and differential diagnosis. It can not only obtain tissue specimens from any part of the body to obtain pathological diagnosis, guide the formulation of treatment plan, prognosis and post-treatment follow-up, but also treat a variety of diseases with minimal damage, avoiding certain surgeries and making up for the shortage of internal medicine treatment.