Nucleic acid testing is done in the pharyngeal isthmus, posterior pharyngeal wall and other parts of the pharynx, and the test requires 3 to 5 examinations. In addition, the pharyngeal swab test should not touch the uvula, oral mucosa, etc. Some people may experience nausea, vomiting and other discomforts. If the pharyngeal swab nucleic acid test is performed, the specific test site is the left or right side of the pharyngeal isthmus, the posterior wall of the pharynx, and the tonsillar crypts. It is recommended that the mucous membrane of the pharyngeal isthmus be repeatedly wiped 3 to 5 times during the nucleic acid test. It is worth noting that contact with the uvula, oral mucous membranes, saliva, and tongue should be avoided during the pharyngeal swab test. Some people may irritate the pharynx when performing the pharyngeal swab nucleic acid test, and some people may experience nausea, vomiting and other discomforts, which are normal phenomena that do not require treatment and will usually disappear on their own. The specific location of the nucleic acid test in the pharynx is the isthmus or throat. It is recommended to avoid overeating, smoking and drinking alcohol or chewing gum before the throat swab test.