The first test for azoospermia in men, and most of the second tests for azoospermia, are also without sperm, mainly due to abnormalities in the reproductive condition that result in sperm not being produced or not being able to be expelled. The main reasons for azoospermia in men are that they do not produce sperm or that the sperm cannot be discharged due to obstruction of the passageway. If the condition is not resolved, the first test for azoospermia and the second test for azoospermia are still basically azoospermia, and further clinical examination is needed to diagnose the specific condition before further treatment. Although the second time is very likely to be azoospermia, it is still necessary to do the second test, and combined with imaging tests, blood tests, etc. to make a comprehensive judgment, to confirm the diagnosis of the condition, and then under the guidance of the clinician to carry out standardized treatment, to avoid causing delays in the condition.