The Sex Hormone 6 needs to be tested twice, once on the 2nd or 3rd day of menstruation and once about 6-8 days after ovulation. The six female sex hormone tests are estrogen, progesterone, follicle stimulating hormone, luteinizing hormone, androgen and lactogen. The first five hormones vary with ovarian function during the menstrual cycle and the timing of the test is determined by the purpose of the test. If the basal hormones of the ovaries are to be checked, the test is usually performed on the 2nd-3rd day of menstruation. If luteal function is to be checked, the test is performed around the 6th-8th day after ovulation. The sex hormone 6 test not only checks whether the ovaries are functioning normally, but also determines whether there is ovulation, normal hormone secretion, and whether there are diseases such as polycystic ovary syndrome. In addition, the hormone 6 test must be performed on an absolutely empty stomach, while ovarian endocrine hormones are easily affected by emotions, mood and diet.