In the IVF cycle, patients with low ovarian function and repeated embryo transfer failures in elderly women are the “biggest problem” among IVF-embryo transfer patients, which is very difficult to deal with! Repeated embryo transfer failures bring heavy psychological and economic burdens to families, and are also the difficulties and research hotspots in the field of assisted reproduction in the world. Recently, the Center for Reproductive Medicine of Changhai Hospital has adopted the method of microstimulation to promote ovulation to obtain frozen embryos and fresh embryos “mix and match” transplantation, which has been repeatedly successful, and has helped the patients with “big problems” to have successful assisted pregnancies. Therefore, this topic is introduced to you. Successful cases (1) A woman, 36 years old, has not been pregnant for 15 years after marriage; her husband has oligozoospermia and weak spermatozoonosis, and he has had two failed intracytoplasmic monosperm injections in foreign hospitals; in September 2015, she had ovulation induction in our center with a short program, and she got 5 eggs and 2 high-quality embryos, and she did not get pregnant after the implantation; in October 2015, she got ovulation induction with microstimulation and she got 2 eggs and 1 effective embryo, and she was frozen for preservation; In November 2015, 2 eggs were obtained from the short program of ovulation promotion, with 1 valid embryo, and this fresh embryo and the frozen embryo in October 2015 were transferred together and successfully conceived, and the ultrasound currently suggests a single intrauterine fetus! (2) A woman, 38 years old, 18 years after giving birth to her first child and 5 years after releasing contraception, was infertile. Her husband had moderate oligospermia, and had failed to conceive with two intracytoplasmic monosperm injections in other hospitals; she had ovulation stimulation with an antagonist regimen in our center in December 2015, and two eggs were obtained, with one valid embryo, which was frozen and preserved; she was again treated with a short course of treatment in our center in January 2016, and the fresh embryo and the frozen embryo in October 2015 were transferred. In January 2016, she ovulated again in our center with the short program and got 1 egg and 1 valid embryo, and then transferred this fresh embryo together with the frozen embryo saved in December 2015 and successfully got pregnant! Why is it good? Explanation: For older women and women with declining ovarian function, the number of follicles available in each IVF cycle may only be 1-2, 1 embryo is obtained, and the success rate of transferring only 1 embryo is low, and due to the effect of clomiphene and the use of microstimulation program to promote ovulation on the lining of the uterus, so it is frozen and stored; in the next second IVF cycle, the use of a shorter program which has a lesser impact on the lining of the uterus and reduces the impact on the tolerance of the uterus, and the use of a shorter program with a lesser impact on the lining of the uterus. In the following second IVF cycle, a shorter protocol with less effect on the endometrium was used to minimize the effect on endometrial tolerance, and the transfer of fresh embryos from the current cycle together with frozen embryos from the microstimulation cycle significantly improved the success rate of embryo transfer in patients with ovarian insufficiency.