Lymph node tuberculosis requires a biopsy to confirm the diagnosis. Obtaining lymph node tissue for pathologic examination is an important basis for confirming the diagnosis of lymph node tuberculosis and is known as the “gold standard”. It is difficult to confirm the diagnosis of lymphatic tuberculosis in one way, and requires a comprehensive analysis of clinical manifestations, physical signs, immunological examinations, imaging examinations (ultrasound, CT, magnetic resonance, etc.), pathological examinations, etc. It is recommended to focus on tuberculosis infection T-cell spotting test and pathological examinations among the above diagnostic methods, which have a small rate of misdiagnosis at the present time. Lymph node biopsy and resection is a method to take lymph node tissues for pathological examination, which is effective, and it is recommended to do biopsy directly from the point of view of accuracy and efficiency. It is recommended to follow the doctor’s instructions to carry out relevant examinations and treatment after detecting lymph node tuberculosis to avoid delaying the condition.