Matters that should be paid attention to in the process of coliform testing include aseptic operation, control experiments, selection of diluent and so on.
1. Aseptic operation: In the process of coliform testing, in order to prevent other bacteria from contaminating the test samples, aseptic operation must be strictly practiced to ensure that the test results are not distorted.
2. Control experiment: when testing coliform bacteria, there should be a negative control group, the purpose is to exclude the experiment false positive, to ensure the authenticity of the experimental results.
3. Selection of diluent: choose the appropriate diluent, such as phosphate buffer can be better protection of the flora to be detected, the sensitivity is also higher.
In addition, the detection process should also pay attention to the incubation time, PH, temperature and other effects on the test results. You should consult a medical professional for the precautions to be taken when testing for coliforms.