False-positive antinuclear antibodies are recommended to be tested several times to clarify whether it is a false-positive or not, and to be diagnosed in conjunction with clinical symptoms and relevant medical history. Antinuclear antibody is one of the tests for autoimmune diseases. A low titer positive antinuclear antibody test may be due to an immune disease, or it may be due to a normal elderly person or a patient with other diseases such as a tumor. In case of false positives, it is recommended that the test be repeated several times to clarify whether the results are false positives or not. Autoimmune diseases require not only antinuclear antibody tests, but also the results of other ancillary tests and the patient’s clinical manifestations, disease history and family history for a comprehensive diagnosis. If the clinical manifestations of autoimmune diseases appear, active treatment is needed to avoid aggravation of the disease. Patients with false-positive antinuclear antibodies are advised to go to the hospital in time for examination, clear diagnosis and active treatment.