As a screening tool for new coronaviruses, nucleic acid testing has been pushed into the public eye, so what exactly is nucleic acid testing? The substance of nucleic acid testing is the nucleic acid of the virus. The nucleic acid test looks for the presence of nucleic acids of foreign invasive viruses in the patient’s respiratory specimen, blood or stool to determine if the patient is infected with a new coronavirus. Therefore, a “positive” test for nucleic acid is evidence of the presence of the virus in the patient’s body. When a new coronavirus infects the body, it first multiplies in the respiratory system, so it can be determined by testing sputum and nasopharyngeal swabs for viral nucleic acids. Therefore, a positive nucleic acid test can be used as the gold standard for confirming the diagnosis of novel coronavirus infection. Now the epidemic prevention and control is still very serious, whether it is school or work, many schools and units are asking for negative results report card. 1. A positive test means that the person is infected with the new coronavirus, and a negative test means that the person is not infected. Nucleic acid testing is performed under laboratory conditions by analyzing the DNA or RNA genetic sequences of disease-causing microorganisms to confirm the clinical etiology of the disease. 2. This nucleic acid test is performed using PCR amplification, which requires many steps and can take up to 6 hours to produce results. This test is highly sensitive, costly, completely manual, and requires a very high workload, as well as very high laboratory conditions. It is currently the most effective test for early determination of whether a patient is infected with the virus, and for early detection and early treatment.