Syphilis testing focuses on checking whether the body is infected with the disease syphilis. It generally includes non-syphilis spirochete antigen serologic test, syphilis spirochete antigen serologic test, dark-field microscopy or nucleic acid amplification test. Syphilis is a sexually transmitted disease (STD) caused by the infection of spirochete pallidum, which is mainly transmitted through sexual transmission, mother-to-child transmission and blood transmission. Generally, 2 to 4 weeks after syphilis infection, a small amount of secretion is taken from the place where the chancre appears for dark-field microscopy or nucleic acid amplification test, which can be positive. After 4 weeks of syphilis infection, a serologic test for syphilis may be positive. If the patient has a history of high-risk sexual contact, there is a risk of syphilis infection, you need to go to the hospital in time to carry out the appropriate examination, sexual partners should also be examined at the same time, both should follow the doctor’s instructions to standardize the treatment, and to avoid sexual intercourse during the treatment.