Tests for Mycoplasma pneumoniae and Chlamydia can determine whether there is an infection of these two pathogens, thus guiding the use of medication, which can use macrolides such as erythromycin. Mycoplasma pneumoniae and Chlamydia can cause acute respiratory tract infections such as acute bronchitis and pneumonia, etc. Serum specific antibody testing can be used to determine whether there is an infection of these two pathogens. The results of Mycoplasma pneumoniae and Chlamydia pneumoniae can help to guide clinical medication. Commonly used penicillin and cephalosporin drugs are ineffective against Mycoplasma pneumoniae and Chlamydia, but macrolide antibiotics such as erythromycin and azithromycin are more effective. Therefore, if the examination is clear that the infection is Mycoplasma pneumoniae and Chlamydia, azithromycin and other drugs can be preferred, in addition to quinolones such as moxifloxacin, tetracyclines such as doxycycline are also effective.