Leaving 24-hour urine protein less than one urine may result in a low urine protein measurement. The method of 24-hour urine protein quantification is that the first urine in the early morning is not to be taken, starting from the second, to the first urine at the same time of the next day, all the urine discharged is collected within a large container, and after mixing it well, 10 ml of it is taken and sent for testing, and the 24-hour urine volume is indicated on the delivery slip. The 24-hour urine protein quantification is the product of the concentration of urine protein and the 24-hour urine volume; therefore, if the patient retains less than one urine volume, this may result in a low 24-hour urine protein quantification. However, protein is not excreted every second, and the amount of protein excreted per second is usually different. If the patient has under-retained urine once in the 24-hour urine protein quantification, it is recommended that the patient go to a regular hospital to have the test results re-prescribed and reassessed by a professional doctor.